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Osaka Prefecture (大阪府, Ōsaka-fu, pronounced [oːsaka ɸɯ]) is a prefecture of Japan located in the Kansai region of Honshu. Osaka Prefecture has a population of 8,778,035 (as of 1 April 2022) and has a geographic area of 1,905 square kilometres (736 sq mi). Osaka Prefecture borders Hyōgo Prefecture to the northwest, Kyoto Prefecture to the north, Nara Prefecture to the southeast, and Wakayama Prefecture to the south.

Osaka is the capital and largest city of Osaka Prefecture, and the third-largest city in Japan, with other major cities including Sakai, Higashiōsaka, and Hirakata. Osaka Prefecture is located on the western coast of the Kii Peninsula, forming the western is open to Osaka Bay. Osaka Prefecture is the third-most-populous prefecture, but by geographic area the second-smallest; at 4,600 inhabitants per square kilometre (12,000/sq mi) it is the second-most densely populated, below only Tokyo. Osaka Prefecture is one of Japan's two "urban prefectures" using the designation fu (府) rather than the standard ken for prefectures, along with Kyoto Prefecture. Osaka Prefecture forms the center of the Keihanshin metropolitan area, the second-most-populated urban region in Japan after the Greater Tokyo area and one of the world's most productive regions by GDP.